创伤对活化T淋巴细胞内IP_3、Ca^（2＋）、CaM及PKC含量的影响

The Effects of Trauma on the Contents of IP_3,Ca^(2+),CaM And PKC in Activated TLymphocytes

利用小鼠闭合伤、截肢伤及烧伤模型，观察创伤后脾脏、胸腺、肠系膜淋巴结中活化Ｔ细胞内ＩＰ＿３、Ｃａ￣（２＋）、ＣａＭ及ＰＫＣ含量的变化。结果显示，创伤后经ＣｏｎＡ活化的Ｔ细胞内ＩＰ＿３、Ｃａ￣（２＋）、ＣａＭ及ＰＫＣ含量均降低；创伤后血清、巨噬细胞、Ｔｓ细胞在体外可明显抑制正常小鼠脾细胞经ＣｏｎＡ活化后的ＩＰ＿３、Ｃａ￣（２＋）、ＣａＭ及ＰＫＣ水平；去除创伤小鼠脾细胞中的巨噬细胞、Ｔｓ细胞可使其细胞内ＩＰ＿３、Ｃａ￣（２＋）、ＣａＭ及ＰＫＣ含量明显提高。提示创伤可导致Ｔ细胞在体外的活化受抑，这一变化同创伤后血清、巨噬细胞及Ｔｓ细胞的作用有关。

urine closed trauma,amputation and burn models were used,the changes of the contentsof IP_3,Ca￣(2+),CaM and PKC in activated T cells from spleen,thymus,mesenteric lymph nodeswere observed. The results showed that the contents of IP_3,Ca￣(2+),CaM and PKC in T cells stim-ulated with ConA were decreased after three types of trauma.The serum,macrophages,Ts cellsfrom traumatized mice could in vitro suppress the levels of IP_3,Ca￣(2+),CaM and PKC in normalsplenocytesstimulated with ConA. Removal of macrophages,Ts cells from splenocytes in trau-matized mice could elevate the contents of IP_3,Ca￣(2+),CaM and PKC significantly.It is suggestedthat trauma can result in depressed T cells activation in vitro,which may be related to the effectsof serum,macrophages and Ts cells post trauma.