小鼠短暂性脑供血不全样发作的实验模型

Study of Transient Ischemia-like Attack in Mice

昆明小鼠尾静脉注射两次枯烯氢过氧化物（ＣＨＰ１∶１０００稀释，０．２０ｍｌ）中间间隔２４ｈ，第二次注射０．５ｈ后，在戊巴比妥麻醉下双侧颈总动脉夹闭１ｈ。１２只实验小鼠在术后２～１２ｈ内不同程度地出现Ｈｏｒｎｅｒ氏征，并出现不同程度的运动功能不良。脑切片ＨＥ染色偶见血栓栓子。仅注射ＣＨＰ组和仅双侧颈总动脉夹闭的对照组均未出现明显的Ｈｏｒｎｅｒ氏征。进一步尾静脉连续注射ＣＨＰ，（１∶１００稀释，０．２５ｍｌ／１００ｇＢＷ）中间间隔２４ｈ，并从第三次开始于每次注射后１５ｍｉｎ用戊巴比妥麻醉后于约０．３Ｌ的容器中窒息１５ｍｉｎ，５４只实验小鼠中有２８只在窒息后不同程度地出现一过性Ｈｏｒｎｅｒ氏征，并有不同程度的运动功能不良。本研究对阐明短暂性脑供血不全样发作（ＴＩＡ）发作的机制有重要意义。

his study is to examine the role of peroxide in the occurrence of transient ischemia-like attack,Cumene hy-droperoxide (CHP 1∶1000,0.25ml/100g body weight)was injected i.v. twice to the 12 Qun-Ming mice with aninterval of 24 hrs between each injection. Half an hour after the second injection ,bilteral carotis communis wereclamped for and hour,These mice were killed 24 hrs after the operation.The paraffin section of brain was madeby routine method. The result showed that all of the 12 mice appeared Horner’s symptom with different degree,4 of them were obvious at right side,7 of them were obvious at left side and 1 of them was doubtful at right side.HE stained section showed plenty of red blood cells were in the brain blood vessels,and there was a thrombus-like material in a section. There was not obvious Horner’s symptom in mice treated only by either CHP or by bi-lateral carotis communis clampping. Further study was conducted as follows : CHP(1∶100) was injceted to mice(0. 2ml/100g body weight)through tail vein with an interval of 24 hrs. From the third session, 15 minutes afterinjection,the mice were anesthetized by pentabarbitol(2.5%,0.1ml)and were suffocated by putting them into acontainer of 0.3 liter for 15 minutes. The result showed that 28 of the 54 mice manifested Horner’s symptomwith different degree. 19 of them displayed this symptom after the first suffocation,and 5 after the second suffo-cation, 2 after the third,another 2 after the fourth suffocation. All of them also showed different kinetic dys-founction. There was a mouse in which the Horner’s symptom lasted for more than 24 hrs. This symptom in oth-er mice disappeared within 24 hrs. However,there were 8 mice which had suspicious infarction area in their brain slices bu TTC staining.