PCR扩增试验的动力学数学模型

The Kinetic Mathematical Model of PCR Amplification Experiment

在认真分析ＰＣＲ技术原理的基础上提出了使用Ｗａｍｐ＝Ｃｕ·Ｍａｍｐ·［Ｎｔａｒｇ·（１＋Ｐ）￣（ｎｌ）＋０．５×Ｃｅｎｚ·Ｕ·Ｐ（ｎ—ｎ＿１）·Ｃｅａｃｔｉｖ—Ｎｔａｒｇ］来估算ＰＣＲ扩增产物数量的动力学方程，并用Ｖｒａｔｅ＝ｄＷａｍｐ／ｄｎ来表示扩增反应产物积累速率的数学模型。实现了对ＰＣＲ反应的定量计算，并可进行给定条件下产物数量，模板数量等参数的预测，有效地指导了ＰＣＲ试验。

n the basis of application of PCR technique theory , we suggested use Wamp = Cu·Mamp(Ntarg· (l +p)￣(nl)+0.5·Cenz·U·p(n n_1)·Ceactiv-Ntarg)to evaluate the kinetic equation ofPCR amplification product quantity and to use Vrate=d Wamp/dn to represent the mathematicalmodel of the accumulation rate of production, We realized the quantitative calculation of PCR re-action and could perform the prediction of some parameters , such as product quantity and tem-plate quantity in given condition. This model can efficiently instruct the PCR experiment.