摘要
离体细胞的非放射性原位杂交单惠敏，黄曼影，王悦，周远征，许荣 （中国医学科学院基础医学研究所，北京１００００５）早在１９６９年，人们就开始把分子杂交技术应用到组织切片和细胞标本上￣［１～３］。现在，虽然在探针标记上大致可把原位杂交分为同位素标记和非同...
e introduced a method of nonradioactive in
situ hybridization of cells in culture in thispaper. The probe is prolactin(PRL )cDNA random
labeled with digoxigenin dUTP. Wesuceessfully detected the change of PRL mRNA in the
anterior pituitary cells in culture treat-ed with some polypeptides,We can observe the effect of a
single factor on the gene transcrip-tion and avoid the influence of other factors with this method.
We also successfully appliedthis method to other cells,such as vessel smooth muscle cells.
Our results indicated that thismethod maybe effective on many kinds of cells in culture.
出处
《基础医学与临床》
CSCD
1995年第5期73-76,共4页
Basic and Clinical Medicine
关键词
离体细胞
细胞培养
原位杂交
细胞杂交
nonradioactive labeling In situ hybridization cells in culture
