激光辐射奶牛冷冻精液对精子诱变效应的研究

EFFECTS OF EH-Ne LASET IRRADIATION ON HOLSTEIN BULLS SPERMATOZOA IN -VITRO FERTILIZATION AND CHROMOSOMAL ABNORMALITIES

为了探索激光辐射精子的诱变效应，本试验采用ＴＳＴ（ＴｒｉｐｌｅＳｔａｉｎＴｅｃｈｎｉｑｕｅ，即精子三重染色法）、ＳＰＡ（ＳｐｅｒｍＰｅｎｅｔｒａｉｔｉｏｎＡｓｓａｙ，即精子穿透分析法）和ＣＡＳ（ＣｈｒｏｍｏｓｏｍａｌＡｎｅｕ－ｐｌｏｉｄｙｉｎＳｐｅｒｍａｔｏｚｏａ，即精子单倍染色体制备技术）三项有关精子的现代分析技术，对激光辐射精子的受精学、遗传学效应进行了研究。结果表明：Ｈｅ－Ｎｅ激光辐射奶牛冷冻精液，可提高精子存活率，促进精子体外获能、发生顶体反应和芽透卵黄膜；具有明显加速雄性原核形成和诱导精子染色体发生畸变的作用。在本试验条件下，经多次预试验确定的辐射时间中，各指标综合效应最佳剂量组为４分组。该组精子顶体反应率、活精子获能率、卵黄膜穿透率、含原核卵子百分率和染色体畸变率比对照组分别增加了９．４５％（Ｐ＜０．０１）、６．８９（Ｐ＜０．０１）、１２．４７％（Ｐ＞０．０５）、１７．６４％（Ｐ＜０．０５）和４６．０３％（Ｐ＜０．０５）。

In order to study the effects of He-Ne Laser Irradiation on Holstein bulls spermfertilizalogy and genetics,frozen semen samples five Holstein bulls were tested by using TST(Triple stain Technique),SPA(Sperm Penetration Assay), CAS(Chromosomal Aneuploidyin Spermatozoa).Our results indicated that He-Ne Laser Irradiation is able to increase therate of survival spermatozoa,and to promote spermatozoa in-vitro capaciation,acrosome re-action, percentage of vitelli penetrated,espesially the development of male pronuclei and fre-quency of spermatozoa chromosomal abnormalities;The rate of true acrosome reaction,sur-vival spermatozoa capaciation,vitelli with male pronuclei and spermatozoa chromosomal ab-normalities of 4 minutes group have increased by 9.45%、6.89%、12.47%、17.64%and 46.03%over the control group respectively.