应用单克隆抗体和免疫斑点试验对B群脑膜炎奈瑟氏菌脂寡糖分型

Serotyping of Lipooligosaccharides of Neisseria Meningitidis Group B with Monoclonal Antibodies and Dot- blotting

本研究使用４种抗Ｂ群脑膜炎奈瑟氏菌（Ｎｍ）脂寡糖的单克隆抗体（ＭｃＡｂ）和免疫斑点试验（ｄｏｔ－ｂｌｏｔｔｉｎｇ）首次对我国不同年代和地区收集的９１株Ｂ群Ｎｍ进行了脂寡糖（Ｌｉｐｏｏｌｉ－ｇｏ－ｓａｃｃｈａｒｉｄｅ，ＬＯＳ）分型，总分型率为９０．１１％，其中Ｌ３．７．９复合型最多，占６３．７４％，其次为Ｌ１．３．７．９复合型，占１２．０９％。该结果为确定我国引起流脑发病的Ｂ群Ｎｍ主要ＬＯＳ型提供了依据，为筛选本国的Ｂ群Ｎｍ菌苗菌株及其作为菌苗成分的ＬＯＳ抗原奠定了基础。

Institute of Epidemiology and Microbiology,Chinese Academy of Preventive Medicine Abstact The serotyping tests of lipooligosaccharides of Neisseria Meningitidis (Nm)group B with 4 monoclonal antibodies and dot-blotting were carried out with the 9 1 strains collected in different decades and areas. Total rate of serotyping is 90. 1 1% . The 63. 74 % of which was L3. 7. 9 com- pound and 12. 09% wasL1. 3. 7. 9 compound,as the first and the second principal strains. The result provides basis for identifying main LOS causing epidemic encephalitis Nm group B , and lays a founda- tion for screening test of Nm group B strain and of their LOS antigen.