蚊血淋巴研究──Ⅱ．马来丝虫微丝蚴感染对中华按蚊血淋巴游离氨基酸的影响

STUDIES ON HEMOLYMPH OF MOSQUITOⅡ.EFFECT OF MICROFILARIAE INFECTION OF BRUGIA MALAYI ON THE FREE AMINO ACIDS IN HEMOLYMPH OF ANOPHELES SINENSIS

应用ＬＣ－６Ａ高效液相色谱仪（ＨＰＬＣ）检测非感染与感染马来丝虫微丝蚴中华按蚊的血淋巴，均测出２０种游离氨基酸。经统计处理这些氨基酸的变化为：１．非感染中华按蚊的氨基酸总量于血餐后第５天比刚羽化后来吸血蚊增加３７％，以后一直维持在增加水平，但第１２天增加量稍减为２１９％。感染马来丝虫中华按蚊的氨基酸总量于血餐后第５天锐减２５．９％，第１２天又增加到２９．０％。２．两组蚊各种氨基酸变化多与其总量变化一致。而感染蚊与非感染蚊比较，则有１５种氨基酸含量具显著性差异（Ｐ＜０．０５），①谷氨酰胺、酪氨酸于血餐后１２ｄ前一直明显增加，甘氨酸＋苏氨酸、蛋氨酸于血餐后１２ｄ量显著增加；天门冬氨酸血餐后５ｄ量减少，１２ｄ也增加。②谷氨酸、丝氨酸、精氨酸、丙氨酸、缬氨酸、亮氨酸、赖氨酸血餐后１２ｄ前量均减少。③鸟氨酸血餐后５ｄ消失，１２ｄ又复出现，苯丙氯酸血餐后１２ｄ前一直未检出。

Twenty kinds of free amino acids (FAA) in hemolymph of Anopheles siensis infected and uninfected with Brugia malayi (BMIAs and UIAs) were detected with LC-6A High Performance Liquid Chromatograph(HPLC) and their regular variations on amount were obserVed. 1.The amount of total FAA:The total FAA of UIAs increased 37% on the 5th day after blood meal,and then decreased slightly (about 21.9 %) around day 12.The total FAA of BMIAs reduced 25.9% on the 5th day and increased 29% on the 12th day. 2.The changes of individual FAA of BMIAs were different from those of UIAs(P<0.05):a. Gln and Tyr increased continuously until the 12th day. G+T and Met increased only on day 12.Asn decreased on day 5 and increased on day 12 too.b. Gln,Ser、Arg,Ala,Val,Leu,Lys reduced continuously until the 12th day.c,Orn dropped to zero on day 5 and reappeared on day 12,Phe kept on decreased and got to zerobefore the 12 th day.