摘要
应用犬蛔虫(T.carnis)的感染性虫卵口饲感染C3H/HeN鼠,体外培养后观察脾细胞的免疫学变化。感染第1-4周的脾细胞用ConA刺激,T细胞的增殖反应,IL-2的诱生均明显地受到抑制,而且感染鼠脾细胞抑制了正常鼠脾细胞对ConA的反应。感染鼠的脾细胞经SephadexG-10过柱后,T细胞则不显示被抑制作用。表明影响T细胞被抑制作用的是感染鼠的巨噬细胞。相反,感染第1-4周的鼠所产生的IgG和IgM等抗体的B细胞活性增强;用LPS刺激巨噬细胞诱生的白介素(IL)IL-1也增多。
C3H/He mice were infected with T.canis by oral administration of embryonated eggs and thier immune functions were studied in vitro. The spleen cells of infected mice failed to respone to T cell mitogen,Con A,but not to B cell mitogen,LPS.Not only proliferative responses but also the production of IL-2 was impaired in spleens from the infected mice.The spleen cells from the infected mice suppressed the Con A response of spleen cells from normal mice, suggesting that suppressor eells develop in the infected mice.The suppressor cells are macrophages because sephadex G-10-purified spleen cells could not show suppressor activity.On the contrary,B cell activity such as Ig-producing cells was enhanced in spleens from the infected mice.Both IgM-and IgG-producing cells were enhanced.The production of IL-1 by macrophages was also enhanced in spleens from the infected mice. All these results suggest that B cell and macrophage activitives are enhanced and T cell activity is suppressed due to the presence of suppressor macrophages in the T.canis-infected mice.1.Dept.Parasito
出处
《寄生虫与医学昆虫学报》
CAS
1995年第2期91-96,共6页
Acta Parasitologica et Medica Entomologica Sinica
关键词
犬蛔虫
白细胞介素
免疫调节
巨噬细胞
Toxocara canis,Interleukine,Immunoregulation,Macrophages