PCR扩增利什曼原虫kDNA动物实验研究对内脏利什曼病早期诊断的价值

DETECTION OF PCK AMPLIFIED kDNA FRAGMENTS IN THE INFECTED GOLDERN HAMSTER TISSUES AND ITS VALUE FOR EARLY DIAGNOSIS OF VIACERAL LEISHMANIASIS

本文报道了用ＰＣＲ扩增Ｌ．ｄｏｎｏｖａｎｌ种特异性ｋＤＮＡ片段来检测不同时间采集的感染金地鼠组织样品，结果表明在腹腔接种感染后第４天就可以从金地鼠外周血和脾组织内检测到病原体ｋＤＮＡ的存在，感染后第４８天，５只金地鼠的外周血和脾组织样品ＰＣＲ检测结果都为阳性，扩增产物经与地高辛标记的重组质粒ｐＬＫ２探针斑点杂交得到进一步证实。由此可见，用ＰＣＲ技术检测外周血或肝、脾组织进行内胜利什曼病的早期诊断是可行的。

For the aim of early diagnosis of visceral leishmaniasis,the specimens collected at different time from the infected goldern hamsters and controls were detected by PCR amplification using the primers Ⅰand Ⅱ.The time of kDNA being detected is as early as 4 days postinoculation peritoneally with the amastigotes of L.donovani(MHOM/CN/92/SCm13), up to 48 days,the spleen and peripheral blood specimens from the 5 infected goldern hamsters were detected and showed all positive after electrophoresis and dot blot hybridization.This study showed that early diagnosis of VL by detecting the PCR amplified kDNA products in spleen、liver specimens and especially in peripheral bolld is full of promising.