微杆菌ZD-M2降解二苯并噻吩的特性及其生长条件优化

Selective-desulfurization of dibenzothiophene by Microbacterium sp. ZD-M2 and optimization of growth conditions

分离得到的微杆菌(Microbacteriumsp.)ZDM2是一株专一性脱硫菌,GCMS分析表明该菌能选择性地脱除二苯并噻吩(DBT)中的硫,沿“4S途径”代谢的终产物2羟基联苯(2HBP)会进一步氧化为2甲氧基联苯(2MBP),同时降解产物中还有联苯(还原产物)存在.在水溶液中降解DBT的结果表明:该菌的适宜生长pH范围为6.5～9.5,最适生长温度为30℃,氯化铵为最佳氮源,生长的最佳浓度为1.0g·L-1.该菌能利用多种碳源和硫源进行生长,但以甘油和DBT为最佳,最适浓度分别为2.0g·L-1和0.2mmol·L-1.

A newly isolated strain was identified as Microbacteriurn sp. ZD-M2, which can specifically remove sulfur from the model compound of dibenzothiophene （DBT） . Metabolites were identified by gas chromatography-mass spectrometry, and the results showed that 2-hydroxybiphenyl, the end product of the previously reported sulfur-specific pathway （also called 4S pathway）, was further converted to 2-methoxybiphenyl. Biphenyl was also produced as the deoxidized metabolites. In aqueous phase the strain grew well in the pH range of 6.5 9.5. The optimal temperature for growth was 30 ℃ and the appropriate concentration of ammonium chloride as the source of nitrogen was 1.0 g·L^-1 Glycerol and DBT were selected as the best carbon and sulfur sources for its growth, and the optimal concentrations were 2.0 g· L^-1 and0.2 mmol· L^-1, respectively.