多重耐药绿脓假单胞菌中I类整合子的研究

Integron-mediated multidrug resistance in clinical multi-reisistant Pseudomonas aeruginosa

目的探讨整合子介导耐药在多重耐药绿脓假单胞菌中所起的作用。方法采用纸片扩散法进行药物敏感试验,筛选多重耐药绿脓假单胞菌;应用三维试验法区别β内酰胺酶型别;采用聚合酶链反应(PCR)技术检测耐药菌中的整合子基因,并应用DNA测序研究整合子内携带的耐药基因。结果多重耐药绿脓假单胞菌中整合子检出率为42.4%,所检出整合子共有3种长度即750、1000、2500bp,它们分别携带了aadB、aadA2、aadB、aac6II和PSE1基因。结论整合子参与是形成绿脓假单胞菌多重耐药的重要因素。

Objective To investigate the action of integron-mediated multidrug resistance in clinical multi-resistant Pseudomonas aeruginosa. Methods The strains of multi-resistant Pseudomonas aeruginosa were selected with K-B susceptibility method. The three-dimensional method was taken to differentiate the various beta-lactamases. The integron was determined by PCR with integron-specific primer. DNA sequencing was also used to analyze resistance-related gene in the integron in multi-resistant Pseudomonas aeruginosa. Results The positive rate of integron detection in multi-resistant Pseudomonas aeruginosa was 42.4%. Three kinds of different length integron were found . They were 750bp, 1000bp and 2500bp and aadB, aadA2, aadB, aac6-II and PSE-1 were the resistance-related gene cassette in the integrons. Conclusions Integron took part in mutiple resistance in multi-resistant Pseudomonas aeruginosa. We should pay more attention to integron-mediated horizontal transference of mulidurg resistance to lessen the occurrence of multi-resistant strains.