东北地区不同宿主NDV分离株的系统发育进化分析

Epidemiological and phylogenetic analysis of NDV isolated in northeast China

本研究针对我国东北地区获得高免新城疫(Newcastledisease,ND)抗体鸡群仍然发生ND的情况,对所分离的18株鸡源新城疫病毒(Newcastlediseasevirus,NDV)和1株鸽源NDV的融合蛋白(F)基因(532bp或280bp)高变区片段进行扩增、克隆和测序(GenBank序列号:AY208680-AY208698)。结果表明,各分离毒株之间核苷酸同源性为83.1%～100%。各分离株间的氨基酸同源性为85.5%～100%。系统进化树分析表明,JL-12、HLJ-3与V4株同属于基因型;HLJ-4、JL-14为基因型,其余15株均为基因型。可见目前东北地区ND的流行是由三种不同基因型毒株,老的基因型、型,新的型所引发,但以基因型为主要流行株,这与我国其他地区和世界其他国家的ND流行情况相一致。

In recent years, there occurred non-typical ND in vaccinated chicken flock of northeast china. To have insight into the molecular epidemiology of Newcastle disease virus （NDV） prevailed. 18 NDV strains isolated from chicken and 1 NDV isolated from pigeon using SPF chicken embryos were studied with RT-PCR and subsequence analysis of the region of 255 nucleotides covering the fusion （F）protein cleavage site （GenBank number： AY208680-AY208698）. The genetic similarities among the viruses isolated are 83. 1%-100%, protein similarities among the viruses are 85. 5%- 100%.Phylogenetic tree indicated JL-12,HLJ-3 were of genotype Ⅰ the same as V4; HLJ-4, JL-14 were of genotype Ⅵ. ; Other 15 strains were grouped under genotype Ⅶ. All the results indicated that NDV in northeast china is very complex, Both old genotype Ⅰ , Ⅵ and novel Ⅶ had been isolated and genotype Ⅶ（15/19）were most prevail strains.