超抗原SEB或SEC对T细胞增殖和活化的影响

Effects of staphylococcal enterotoxin B or enterotoxin C on the proliferation and activation of T lymphocytes

目的:探讨超抗原金黄色葡萄球菌肠毒素B(staphy lococcal enterotoxinB,SEB)和金黄色葡萄球菌肠毒素C(staphy lococcal enterotoxinC,SEC)在体外对T细胞增殖和活化的影响。方法:不同浓度SEB或SEC在体外刺激T细胞,采用MTT法检测T细胞增殖水平,在流式细胞仪(flowcytometer,FCM)上检测T细胞受不同浓度SEB或SEC刺激后不同时间其早期活化标志CD69的表达水平。结果:SEB或SEC在体外能明显激活T细胞,以浓度为100μg/L时活化T细胞的作用最强,刺激时间以4～8h为佳,而对T细胞的增殖无明显影响。SEB和SEC活化T细胞的作用强度之间无明显差异。结论:以上结果提示,SEB或SEC具有很强的刺激T细胞活化的能力,为其应用于肿瘤免疫治疗提供了一定依据。

Objective To observe the effects of staphylococcal enterotoxin B （SEB） or staphylococcal enterotoxin C （SEC） on the proliferation and activation of T lymphocytes in vitro. Methods After T lymphocytes were stimulated by different concentrations of SEB or SEC at different stimulating time, the proliferation of T lymphocytes was determined using MTT assay. Then, the expression level of CD69 on T lymphocytes was measured using flow cytometer. Results SEB or SEC could significantly activate T lymphocytes, with 100 μg/L of its being the most efficient dose and with the best stimulating time from 4 h to 8 h, But SEB or SEC had no significant effects on the proliferation of T lymphocytes. There was no significant difference between both SEB and SEC. Conclusion SEB or SEC posses the strong ability to activate T lymphocytes, which provides the foundation for clinical tumor immunotherapy.