实时荧光定量PCR检测丙型肝炎病毒

Detection of Hepatitis C virus RNA by Real-Time Fluorescent Quantitative PCR

目的探讨实时荧光定量PCR(FQ-PCR)技术检测丙型肝炎病毒(HCV)RNA载量与抗-HCV及丙氨酸氨基转移酶(ALT)异常的相关性。方法用FQ-PCR检测115份疑似HCV感染的病人血清HCV-RNA,ELISA法检测抗-HCV,全自动生化分析仪测定ALT。结果以80拷贝/ml血清为标准,115份标本中HCV-RNA阳性率为54.8%(63/115);抗-HCV阳性率为53.9%(62/115);ALT异常率为40.9%(47/115)。HCV-RNA载量与抗-HCV(+)例数呈正相关(r=0.986,P<0.01)。HCV-RNA载量与ALT异常例数(r=0.94,P<0.01)、含量(r=0.624,P<0.01)呈正相关。结论HCV-RNA载量升高,抗-HCV阳性率相应增加,ALT高于正常值(40U/L)的异常率和浓度也增高,均呈正相关。FQ-PCR法可作为临床检测丙型肝炎的确诊方法之一。

Objective To investigate the correlation between the quantity of HCV- RNA （detected by Real- time Fluorescent Quantitative PCR） and concentrations of anti - HCV and alanine transaminase （ALT）. Methods The quantify of HCV-RNA in 115 serum samples were detected by fluorescent quantitative PCR （FQ- PCR）, the concentrations of anti - HCV and ALT were detected by Enzyme immunoassay and automatic biochenaistry analyzer. Results In 63 of 115 serum samples,the quantity of HCV - RAN was more than 80 copies per milliliter （ the positive rate of HCV - RNA was 54.8 % ） ; the positive rate of anti - HCV was 53.9 % （62/115） ; the ALT in 40.9 percent of samples （47/115） was abnormal. It was positive correlation between quantity of HCV- RNA and the number of anti - HCV positive samples （ r = 0. 986, P 〈 0.01）. It was positive correlation between the quantity of HCV- RNA and the number of samples with abnormal ALT （ r = 0.94, P 〈 0.01 ）, and the same between quantity of HCV- RNA and content of ALT（r =0.624, P〈0.01）. Conclusions It is positive correlation between the quantity of HCV- RNA and the positive rate of anti - HCV, abnormal rate of ALT, content of ALT. FQ- PCR can be a method of diagnosing hepatitis C.