慢性乙型肝炎和肝硬化患者血清HA、LN、PCⅢ和Ⅳ-C水平与肝组织纤维化程度的关系

Determination of serum levels of collagen typeⅣ,procollagen type Ⅲ,hyaluronic acid and laminin in patients with chronic hepatitis and cirrhosis

目的探讨慢性乙型肝炎和肝硬化患者血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和Ⅳ型胶原(Ⅳ-C)在肝纤维化诊断中的应用价值。方法采用放射免疫法检测150例慢性乙型肝炎和肝硬化患者血清HA、LN、PCⅢ和Ⅳ-C水平,并与活检肝组织纤维化程度进行相关性分析。结果各组慢性乙型肝炎患者血清HA、LN、PCⅢ和Ⅳ-C水平均高于正常对照组,与肝纤维化活动水平及程度呈密切正相关。结论血清HA、LN、PCⅢ和Ⅳ-C水平能较好地反映乙型肝炎纤维化程度,联合检测可明显提高肝纤维化诊断的准确性和可靠性。

Objective To investigate the changes of serum levels of collagen type Ⅳ（Ⅳ-C）, procollagen type Ⅲ （PC Ⅲ ）,hyaluronic acid （HA） and laminin（ LN）and their relationship with hepatic fibrosis in patients with chronic hepatitis B and cirrhosis. Methotis Serum levels of Ⅳ-C, PC Ⅲ , HA and LN were measured with radioimmtmossay in 150 patients with chronic hepatitis B. Hepatic fibrosis was assessed based on liver biopsy. Results The serum levels of Ⅳ-C,PCⅢ ,HA and LN in patients with chronic hepatitis B of various types were higer than those in control group. Sentm levels of those markers were positively correlated with the degree of pathological changes of fibrosis. Conclusion Serum levels of these markers reflect the degree of hepatic fibrosis and severity of liver damage. Combined determination of those markers is a sensitive and reliable diagnostic method in diaguosing liver fibrosis.