反相高效液相色谱法测定珍黄丸中黄芩苷的含量

Determination of baicalin in Zhenhuang Wan by RP-HPLC

目的:应用高效液相色谱法测定珍黄丸中黄芩苷的含量。方法:色谱柱为Alltima C18(5μm,4.6×150mm),流动相为甲醇-水-冰醋酸(50:50:1),检测波长为273nm,流速为1.0mL/min。结果:黄芩苷在7.4444-37.220μg范围内进样量与峰面积呈良好线性关系(r=0.999 7);平均加样回收率为98.58%,RSD =0.65%(n=5)。结论:本方法测定结果准确、灵敏、重现性好。

Objective：To develop a RP-HPLC method for determination of baicalin in Zhenhuang Wan. Methods： Alltima C18 column was used and methanol-water-acetic acid glacial （50 ： 50 ： 1 ） was used as mobile phase,detection wavelegth was 273nm. Results： There was a good linear relationship between the concentration of baicalin and absorption area value in range of 7. 444 - 37. 220μg r =0. 999 7. The average recovery was 98.55% with RSD =0. 65% （ n =5）. Conclusion：The method was reoeatable and accurate.