人精液中NO与TP、Alb及Tf含量的关系

Correlation between nitric oxide and the contents of total protein,albumin and transferrin in human semen

目的探讨人精液一氧化氮(NO)与总蛋白(TP)、白蛋白(Alb)和转铁蛋白(Tf)含量的关系。方法参照WHO标准方法,进行精液常规分析。采用镀铜镉还原荧光法检测NO代谢产物硝酸盐(NO-3),用双缩脲法检测TP,溴甲酚绿法测定Alb,用免疫散射比浊法检测Tf含量。结果不育活率异常组、少精子和无精子症组的NO含量明显高于正常生育组,TP、Alb和Tf含量明显低于正常生育组,两组之间存在高度显著性差异(P<0.01),不育组NO含量与TP、Alb和Tf含量呈显著性负相关(r=-0.88、r=-0.98、r=-0.68,P均<0.05)。结论精液蛋白质含量测定有助于精子质量的评价,对男性不育症的诊治有一定的指导意义,NO对精子运动能力及蛋白质的分泌利用有抑制作用,这对不育症的机制研究有重要价值。

Objective To discuss the correlation between nitric oxide and with the contents of total protein, albumin and transferrin in human semen. Methods The semen was analyzed according to WHO＇s standard criteria. Copper-coated cadmium reduction fluorescence assay was used to detect the metabolic product of NO nitrate. The contents of TP, Alb and Tf were measured using biurea method, bromocresol green method and immunonephelometry respectively. Results The nitric oxide level in the groups of asthenozoospermia, oligozoospermia and azoospermia was remarkably higher than that in control group, but the conditions of Tp, Alb and Tf are contrary, and there is great difference between two groups.（P〈0.01）. The contents of Tp, Alb and Tf in inferile group were negatively related with NO level （r=-0.88, r=-0.98, r=-0.68, P〈0.05）. Conclusion The protein contents in semen may be helpful to evaluate the quality of sperms, and NO inhibits the activity of sperms and the protein secretion. So they can be utilized in the diagnosis and treatment of male infertility.