葛根素对实验性大鼠电离辐射损伤的保护效应

Protective effects of puerarin on radiation injury of experimental rats

目的 :观察葛根素对实验性大鼠电离辐射损伤的保护效应 ,探讨其保护作用的可能机制。方法 :Wistar大鼠随机分为 4组 ,每组 8只 :生理盐水非辐射 (physiologicalsalinenon radiation ,SN)组 ,葛根素非辐射 (puerarinnon radiation ,PN)组 ,生理盐水辐射 (physiologicalsalineradiation ,SR)组 ,葛根素辐射(puerarinradiation ,PR)组。辐射源为6 0 C0 γ源 ,1次辐射剂量为 18Gy ,流量为 1.2Gy/min。葛根素为注射针剂 ,辐射后连续静脉注射 6d ,每次剂量为 30mg/kg。辐射 1周后取样测定。 结果 :葛根素处理能够有效缓解辐射引起的大鼠外周血红细胞和白细胞的下降 ;减轻辐射大鼠胸腺指数和脾脏指数的下降 ;提高心肌组织超氧化物歧化酶 (superoxidedismutase ,SOD)活性和降低膜脂过氧化产物丙二醛 (malondialdehyde ,MDA)的含量。 结论 :葛根素对实验性大鼠电离辐射损伤有明显的保护效应 ,其保护效应的机制可能与葛根素的抗氧化作用有关。

Objective： To observe the protective effects of puerarin on radiation injury of experimental rats and to discuss the possible mechanism of its radiation protection. Methods： Wistar rats were divided randomly into 4 groups with 8 rats in each group： physiological saline non-radiation （SN） group, puerarin non-radiation （PN） group, physiological saline radiation （SR） group, puerarin radiation （PR） group. The source of radiation was cobalt-60 gamma rays, and the rats were exposed to radiation （ 1.2 Gy/min） at a dose of 18 Gy. Following irradiation of the rats, puerarin was injected intravenously at each dose of 30 mg/kg in 6 consecutive days （24 h interval）. Samples were collected and assayed one week later. Results：Puerarin delayed effectively the declines of the quantity of red blood cells and white blood cells in circulation due to ionizing irradiation, improved the index of thymus and spleen in ionizing irradiation rats, increased the activity of superoxide dismutase （SOD） and decreased the level of malondialdehyde （MDA） in the ratmyocardial tissue. Conclusion. Puerarin has a marked protective effect on ionizing irradiation injury of experimental rats, and its protective mechanism is probably based on oxidation-resistance.