消炎灵口服液中黄芩苷含量的测定

Determination of baicalin in Xiaoyanling Decoction by high- performance liquid chromatography

目的 :检测消炎灵口服液中黄芩苷的含量。方法 :用高效液相色谱法 ,条件采用KromosilC18柱 ,流动相为甲醇 酸水 (水∶磷酸 =5 3∶0 .2 ) 4 0∶6 0 ,检测波长 2 77nm。结果 :各批消炎灵口服液中黄芩苷的含量每毫升均不低于 0 .4 0mg ,RSD <5 %。 结论 :消炎灵口服液中君药黄芩中主要有效成分黄芩苷在制剂中的含量较高 ,且稳定、可靠 ,可用于消炎灵口服液的质量控制。

To establish a high-performance liquid chromatography （HPLC） method for the determination of baicalin in Xiaoyanling Decoction. Methods： HPLC method was used for determination.A Kromosil C18 column was used. The mobile phase consisted of MeOH-acid water （H2O ： H3PO4 =53 ： 0.2）40 ： 60, and the detective wavelength was 277 nm. Results： The content of baicalin in each Xiaoyanling Decoction sample was not less than 0.40 mg/ml （RSD〈5%）. Conclusion. The content of baicalin in Xiaoyanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.