补肾柔肝方治疗大鼠肝纤维化的实验研究

Therapeutic effect of Bushen Rougan Recipe on hepatic fibrosis in rats

目的:探讨中药复方补肾柔肝方对二甲基亚硝胺(dimethylnitrosamine,DMN)诱导的大鼠肝纤维 化的治疗作用。方法:雄性Wistar大鼠40只,随机分为正常对照组、模型组及治疗组。模型组和治疗组大 鼠以10mg/kg的剂量腹腔注射0.5%DMN,1次/d,每周连续3d,共4周。模型组在造模结束后给予生理 盐水灌胃,而治疗组在造模结束后灌胃补肾柔肝方(10ml·kg-1·d-1)进行治疗干预,共用药4周。第8周 末处死全部大鼠,采用全自动生化分析仪检测血清总胆红素(totalbilirubin,TBIL)、丙氨酸氨基转移酶 (alanineaminotransferase,ALT)、天冬氨酸氨基转移酶(aspartateaminotransferase,AST)、白蛋白 (albumin,A)及球蛋白(globulin,G)等;用放射免疫法检测血清透明质酸(hyaluronicacid,HA)、层黏连蛋 白(laminin,LN)及Ⅳ型胶原(collagenⅣ,Ⅳ C)的含量。结果:治疗组大鼠一般状态明显好于模型组;正常 组大鼠无死亡,而模型组大鼠病死率为40%(6/15),治疗组病死率为20%(3/15);与正常组比较,模型组大 鼠的血清TBIL、ALT及AST显著升高(P<0.01),治疗组较模型组有显著改善(P<0.01);模型组大鼠 血清的A/G严重倒置,达到0.62±0.07,较正常组差异显著(P<0.01),而治疗组的A/G有一定程度的改 善,为0.77±0.04(P<0.05),但是仍倒置比较明显。血清胶原?

Objective： To study the therapeutic effect of Bushen Rougan Recipe （BSRGR） on hepatic fibrosis in rats. Methods： Forty male Wistar rats were randomly divided into normal control group （n = 10）,model group （n = 15）, and BSRGR-treated group （n = 15）. Rats in the model and BSRGR-treated groups were administered intraperitoneally with 0.5% dimethylnitrosamine （DMN）, 10 mg · kg^-1 · d^-1, successive 3 days per week for 4 weeks to induce hepatic fibrosis. Then rats in the above 2 groups were given normalsaline and BSRGR （10 ml · kg^-1· d^-1 , ig） for another 4 weeks, respectively. Rats in the 3 groups were all executed at the end of the 8^th week. The serum total bilirubin （TBIL）, alanine aminotransferase （ALT）,aspartate aminotransferase （AST）, albumin （A） and globulin （G） were determined and the serum hyaluronic acid （HA）, laminin （LN） and collagen Ⅳ （Ⅳ-C） were measured. Results： The rat model of liver fibrosis was successfully induced by DMN.It was found that the serum TBIL, AST and ALT and the liver fibrosis marks were declined in BSRGR-treated group as compared with those in the model group （ P〈0. 01 ）. The content of total serum protein and the A/G in BSRGR-treated group were both increased as compared with those in the model group （P〈0.05）. Conclusion： BSRGR can be used to treat hepatic fibrosis in rats.