摘要
目的:探讨中药复方补肾柔肝方对二甲基亚硝胺(dimethylnitrosamine,DMN)诱导的大鼠肝纤维 化的治疗作用。方法:雄性Wistar大鼠40只,随机分为正常对照组、模型组及治疗组。模型组和治疗组大 鼠以10mg/kg的剂量腹腔注射0.5%DMN,1次/d,每周连续3d,共4周。模型组在造模结束后给予生理 盐水灌胃,而治疗组在造模结束后灌胃补肾柔肝方(10ml·kg-1·d-1)进行治疗干预,共用药4周。第8周 末处死全部大鼠,采用全自动生化分析仪检测血清总胆红素(totalbilirubin,TBIL)、丙氨酸氨基转移酶 (alanineaminotransferase,ALT)、天冬氨酸氨基转移酶(aspartateaminotransferase,AST)、白蛋白 (albumin,A)及球蛋白(globulin,G)等;用放射免疫法检测血清透明质酸(hyaluronicacid,HA)、层黏连蛋 白(laminin,LN)及Ⅳ型胶原(collagenⅣ,Ⅳ C)的含量。结果:治疗组大鼠一般状态明显好于模型组;正常 组大鼠无死亡,而模型组大鼠病死率为40%(6/15),治疗组病死率为20%(3/15);与正常组比较,模型组大 鼠的血清TBIL、ALT及AST显著升高(P<0.01),治疗组较模型组有显著改善(P<0.01);模型组大鼠 血清的A/G严重倒置,达到0.62±0.07,较正常组差异显著(P<0.01),而治疗组的A/G有一定程度的改 善,为0.77±0.04(P<0.05),但是仍倒置比较明显。血清胶原?
Objective: To study the therapeutic effect of Bushen Rougan Recipe (BSRGR) on hepatic fibrosis in rats. Methods: Forty male Wistar rats were randomly divided into normal control group (n = 10),model group (n = 15), and BSRGR-treated group (n = 15). Rats in the model and BSRGR-treated groups were administered intraperitoneally with 0.5% dimethylnitrosamine (DMN), 10 mg · kg^-1 · d^-1, successive 3 days per week for 4 weeks to induce hepatic fibrosis. Then rats in the above 2 groups were given normalsaline and BSRGR (10 ml · kg^-1· d^-1 , ig) for another 4 weeks, respectively. Rats in the 3 groups were all executed at the end of the 8^th week. The serum total bilirubin (TBIL), alanine aminotransferase (ALT),aspartate aminotransferase (AST), albumin (A) and globulin (G) were determined and the serum hyaluronic acid (HA), laminin (LN) and collagen Ⅳ (Ⅳ-C) were measured. Results: The rat model of liver fibrosis was successfully induced by DMN.It was found that the serum TBIL, AST and ALT and the liver fibrosis marks were declined in BSRGR-treated group as compared with those in the model group ( P〈0. 01 ). The content of total serum protein and the A/G in BSRGR-treated group were both increased as compared with those in the model group (P〈0.05). Conclusion: BSRGR can be used to treat hepatic fibrosis in rats.
出处
《中西医结合学报》
CAS
2005年第2期132-135,共4页
Journal of Chinese Integrative Medicine
关键词
补肾柔肝方
肝纤维化
二甲基亚硝胺
动物
实验
Bushen Rougan Recipe
hepatic fibrosis
dimethylnitrosamine
animals, laboratory