龙葵碱对荷瘤小鼠肿瘤细胞DNA和RNA的影响

Effect of solanine on DNA and RNA in tumor cell of tumor-bearing mice

目的观察龙葵碱对S180和H22荷瘤小鼠肿瘤细胞DNA和RNA的影响。方法将S180和H22荷瘤小鼠分为龙葵碱37.50、18.75、9.37 m g/kg组、阴性对照组和环磷酰胺(30 m g/kg)组,各组sc给药。利用激光共聚焦扫描显微镜,分别测定各组肿瘤细胞中的DNA和RNA。结果龙葵碱(37.50、18.75 m g/kg)组对S180和H22小鼠肿瘤细胞RNA和DNA的比值均有明显的降低作用。结论龙葵碱降低S180和H22小鼠肿瘤细胞RNA和DNA的比值可能是其抗肿瘤作用机制之一。

Objective To observe the effects of solanine on DNA and RNA level in tumor cell of S180 and H22 tumor-bearing mice. Methods S180 and H22 mice were divided into solanine （37.50, 18.75, and 9.37 mg/kg） groups, negative control group and cytoxan （30 mg/kg） group, whom were given drugs by sc. Levels of RNA and DNA in tumor cell membrane in every groups were examined, respectively by laser scanning confocal microtechnic technology. Results Solanine （37.50,18.75 mg/kg） groups reduced the ratio of RNA and DNA in tumor cell of both S180 and H22 mice. Conclusion Solanine can reduce the ratio of RNA and DNA in tumor cell of S180 and H22 mice, which may be one of the mechanisms of solanine＇s antitumor effect.