壳聚糖诱导番茄抗青枯病的作用

Tomato resistance to Ralstonia solanacearum induced by chitosan

为了探索防治番茄青枯病的有效途径,我们对接种青枯病菌后的番茄进行40mg/L 浓度壳聚糖的不同方式处理。结果表明,喷施壳聚糖可诱导番茄对青枯病产生抗性,减轻青枯病病情,经二次喷施后再喷微量元素的处理相对防效达到48.76％。体内与抗病反应有关的苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性峰值分别比对照高46.24％、51.77％、121.22％、36.49％。同时壳聚糖处理的番茄叶片中叶绿素含量明显高于正常接菌植株。

To search an effective approach to preventing tomato bacterial wilt, chitosan were sprayed in tomato inoculated with Ralstonia solanacearum, to study effect of chitosan on bacterial wilt disease and resistance mechanism. The results indicated that chitosan spray induced tomato resistance to bacterial wilt and reduced disease index. The relative cure effect of the treatment 3, in which chitosan was sprayed twice and jiduojing once, reached 48.76%. The peak value of activities of four enzymes, i.e. , phenylanine ammonia lyase （ PAL ） , polyphenoloxidase （ PPO ）, peroxidase （ POD ） and superoxide dismutase （SOD） ,related to resistance in treatment 3 increased respectively by 46.24%, 51.77%, 121.22%, 36.49%. The contents of chlorophyll in leaves of tomato after chitosan spray were significantly higher than those of normal inoculation tomato.