应用反义转化生长因子基因进行骨肉瘤基因治疗的研究

Study on the gene therapy for osteosarcoma by antisense TGF-β1 gene

目的探讨反义转化生长因子β1基因治疗骨肉瘤的价值。方法用转基因技术将反义转化生长因子基因导入骨肉瘤细胞LM-8,构建转基因细胞株。用骨肉瘤细胞株LM8皮下注射C3H雄性小鼠建立小鼠骨肉瘤移植瘤模型。应用裸反义转化生长因子基因、灭活的转基因骨肉瘤细胞分原位和异位进行治疗。结果两种治疗措施都可表现出抑瘤作用,以灭活的转基因骨肉瘤细胞治疗效果最佳,原位治疗效果优于异位治疗。结论反义转化生长因子基因对小鼠移植性骨肉瘤有较好的实验治疗效果,为人骨肉瘤进行反义转化生长因子基因治疗提供了一定的依据。

Objective To intesvigation the effects of treatment off osteosarcoma by antisense TGF- β1 gene in vivo, Methods LM8s were transfected with antisense TGF- β1 gene through lipofectamine vector and were selected by G418. Nude antisense TGF - β1 gene was mixed with lipofectamine.The model of ostecsareoma was established in C3h mice injected with LM8 lines, The model tumor was treated by inactivated transduced LM8 and pcDNA3 - anti - TGFβ1 mixtures at of original site and different site. The control group was treated with PBS, Results Significant therapeutic effects was achieved by the treatment of inactivated transduced LM8 and pcDNA3 - anti - TGFβ1 mixtures. Strong inhibition to tumor growth was found. A better anti-tumor effect was achieved at original site tumor compared with tumor at different site. Conclusion Gene therapy for osteosarcoma by antisense TGF- β1 gene is feasible and effective, providing the foundation of gene therapy for human osteosarcoma.