肝素酶抑制剂对食管癌移植瘤中uPA VEGF蛋白表达的影响

The Effect of Heparanase Inhibitor Laminarin Sulfate on the Expression of uPA, VEGF in the Esophageal Carcinoma Carried by Nude Mice

目的:探讨肝素酶抑制剂硫酸多糖体内对裸鼠食管癌移植瘤中与肝素酶作用相关uPA、VEGF蛋白表达的影响。方法:硫酸多糖分组处理人食管癌EC9706细胞后,进行裸鼠体内成瘤实验,采用免疫组化SP法检测裸鼠食管癌移植瘤中uPA、VEGF的蛋白表达。结果:4种不同浓度的肝素酶抑制剂硫酸多糖对裸鼠食管癌移植瘤中uPA、VEGF蛋白表达均有明显的抑制作用,实验组间两两相比,差异无统计学意义(P>0.05);与不加硫酸多糖的对照组两两相比,差异有统计学意义(P<0.05)。结论:硫酸多糖可抑制裸鼠食管癌移植瘤中uPA、VEGF蛋白表达,说明硫酸多糖对肿瘤浸润、转移有一定的抑制作用。

Objective： To investigate the effects of heparanase inhibitor laminarin sulfate on the expression of heparanase relevance gene uPA, VEGF in the tumor carried by nude mice. Methods： After EC9706 cells were disposed by four concentrations laminarin sulfate （0.2μ/ml, 0.4μ/ml, 0.6μ/ml, 0.8μg/ml） for 24 hours, they were injected into nude mice, the protein expression of heparanase relevance gene uPA, VEGF in the tumor carried by nude mice were observed by using immunohistochemistry SP method. Results： The expression of uPA and VEGF proteins in transplanted tumor was inhibited by four concentration inhibitor laminarin sulfate, especially 0.6μg/ml, the inhibitory effect on VEGF was stronger compared to that on uPA, but there was no significant difference between them （P〉0.05）. There was a significant difference between control group and experimental group （P〈0.05）. Conclusion： Observation shows that laminarin sulfate can inhibit the expression of uPA and VEGF protein in tumors carried by nude mice, indicating that laminarin sulfate is an effective inhibitor for invasion and metastasis of tumors and can provided a new clue for preventing invasion and metastasis of tumors clinically.