摘要
目的:体外观察紫杉醇及抗HER2单抗对人卵巢癌细胞株A2780增殖的抑制作用及两者联合应用时的抑制率及相互作用。方法:采用MTT法测定两者单用或合用对A2780的增殖抑制率,利用中效原理判定两药合用的效果,用流式细胞仪观察细胞凋亡及细胞周期的变化。结果:两种药单独应用时随药物剂量增加,其效应也增加,紫杉醇的中效浓度为6．98 nmol· L-1,抗HER2单抗的中效浓度为8．69μg·ml-1;两药合用时它们的中效浓度为2．92 nmol·L-1+0．49μg·ml-1。大剂量(紫杉醇>13．02 nmol·L-1,抗HER2单抗>2．17 μg·ml-1)合用时是拮抗作用(CI>1),小剂量(紫杉醇<13．02 nmol·L-1,抗HER2 单抗<2．17 μg·ml-1)合用时是协同作用(CI<1)。结论:紫杉醇联合抗HER2单抗对人卵巢癌细胞株A2780的生长有抑制作用,它们合用时大剂量是拮抗,而小剂量则为协同作用。
Objective： To observe the inhibiting effects of paclitaxel （TAX） and anti HER2 mAb on human ovarian cancer cell strain A2780 and the inhibiting rate and interaction when the two drugs were used together. Method： The inhibition of TAX and anti HER2 mAb on A2780 cells used together or separately was measured by MTT assay. The interaction effect of two drugs was analyzed with median-effect principle. The changes of cell period and the apoptosis were observed by using flow cytometer （FCM）. Result： The effects of the individual drugs were enhanced as drug dosage increased. The median concentration of Tax was 6.98 nmol·L^-1 and that of anti-HER2 mAb was 8.69 μg·ml^-1, The median concentration of combination of two drugs was 2.92 nmol·L^-1 ＋ 0.49 μg·ml^-1.The interaction of two drugs was antagonistic at higher concentration （Concentration of Tax 〉 13.02 nnmol·L^-1, Concentration of antiHER2 mAb 〉 2.17μg·ml^-1 ） （ CI 〉 1 ） and synergistic at lower concentration （ Concentration of Tax 〈 13.02 nnmol·L^-1, Concentration of anti-HER2 mAb 〈 2. 177μg·ml^-1） （ CI 〈 1 ）. Conclusion ： The united effect of the Tax and anti - HER2 mAb on A2780 cells is antagonistic at higher concentration and synergistic at lower concentration.
出处
《中国药师》
CAS
2005年第8期623-625,共3页
China Pharmacist
基金
国家自然科学基金资助项目(No.30300429)
