高效液相色谱法测定2,3-双脱氧肌苷的含量

Determination of Didanosine by HPLC

目的:建立测定2,3-双脱氧肌苷含量及相关物质的高效液相色谱法。方法:采用Aglient CLC-ODS色谱柱。醋酸铵缓冲液:甲醇=85:15为流动相,流速为1．0 ml·min-1,检测波长:254 nm。结果:2,3-双脱氧肌苷在14-25 ug·ml-1浓度范围内,与其峰面积呈良好的线性关系,r=0．999。平均回收率为100．2％,RSD0．4％。结论:方法可靠,简单可行。

Objective： To set up a method for determining the content of didanosine and the related substances by HPLC. Method： Aglient CLC-ODS column was used. The ammonium acetate buffer solution-methanol （85：15 ） as mobile phase. The flow rate was 1.0 ml·min^- 1. The detection wavelength was 254 nm. Result： The calibration curve presented a good linear within the range of 14 to 25 μg·ml^ -1 （ r = 0.999）. The average recovery was 100.2% （ RSD = 0.4% ）. Conclusion： This method is accurate and reliable.