持续癫痫大鼠行为学和神经肽Y mRNA表达的改变

Changes in behavior and neuopeptide Y mRNA expression of sustain epileptic rats

目的探讨癫痫持续状态(SE)对大鼠情感行为和学习记忆功能的影响及海马组织神经肽Y(NPY)mRNA的表达变化。方法戊四氮诱导大鼠SE,采用抬高迷宫和Morris水迷宫观察大鼠情感反应和学习记忆功能的改变。反转录多聚酶链反应(RT-PCR)方法检测大鼠海马NPY mRNA的表达。结果SE组大鼠在抬高迷宫开放臂中逃避时间延长(P<0.01),进入次数增多(P<0.01);水迷宫中逃避潜伏期延长(P<0.01),搜寻策略变差(P<0.05),平台象限游泳时间百分比降低(P<0.01),穿越平台次数减少(P<0.01)。同时伴有海马NPY mRNA表达上调(P<0.05)。结论SE可使大鼠情感行为改变和学习记忆功能受损,NPY可能参与这一变化的病理生理过程。

Objective To explore the effect of status epilepticus（SE） on their emotional behavior, spatial learning and memory, and its molecular mechanism. Methods Forty adult male SD rats, weighing（200 ± 20）g were randomly divided into SE group（SG）. Pentylenetetrazole（PTZ） and normal control group（NG）. The change of emotional behavior in two groups were tested in Elevated plus maze. Furthermore, Morris water maze was applied to evaluate the affection of SE on spatial learning and memory of rats. At the same time, The expression of neuropeptide Y（NPY） mRNA in hippocampus was determined by reverse transcription polymerase chain reaction（RT-PCR）. Results In elevated plus test, SE rats increased the times of visits as well as the time spent on the open arms of the elevated plus maze （ P 〈 0.01 ）. In Morris water maze, the mean escape latency for the SE rats looking for hidden platform in the place navigation test prolonged（ P〈 0.01） ; the efficiency of their search strategy was poor（ P 〈 0.05） ; the swimming time in platform region and the percentage of their swimming time decreased（ P 〈 0.01 ） ; the number of times they crossed the platform area decreased（ P 〈0.01 ） ; Meanwhile the expression level of NPY mRNA in hippocampus was increased （ P 〈 0. 05）. Conclusion The resuhs showed that SE could result in the change of emotional behavior and damage of spatial learning and memory of rats.NPY might be involved in the patho- and physiological process.