1级杂系昆明小鼠CagA^+VacA^+Hp感染模型的建立

Establishment of Helicobacter pylori infection model in crossbred Kuming mice

目的:建立稳定、可靠的CagA+VacA+Hp1级杂系昆明小鼠感染模型,观察感染小鼠的胃粘膜病理改变。方法:昆明小鼠随机分成4组(n=6),经胃给40%乙醇0.2ml,禁食12h,模型一、二组经胃给菌液0.5ml/只(浓度为109CFU/ml),对照一、二组给生理盐水,每间隔12h重复一次,共三次。另设置1组作为对照三组(n=6),不作任何处理。距末次感染后的第4周和第8周分别处死小鼠,取胃粘膜组织标本进行涂片检查、尿素酶试验、常规病理学HE染色和WS染色。结果:模型一、二组感染率为100%。所有感染动物胃粘膜细胞表面和胃小凹有Hp定植,固有层出现慢性炎症细胞的浸润,模型二组出现腺体萎缩。结论:高浓度(1×109CFU)SS1多次经口感染1级昆明杂系小鼠可制备稳定、可靠的幽门螺杆菌感染动物模型,感染动物胃粘膜组织有SS1定植并出现明显的炎症反应。

Objective： to establish a stable and reliable model of H. pylori infection in crossbred Kuming mice and to observe pathological changes in gastric mucosa from the infected animals. Methods： Crossbred Kuming mice were randomly divided into 4 groups（ n=6）,which were challenged 40% alcohol 0.2ml by gastric and no feeding 12h. The mice of experimental groups were challenged with 0.5ml H. pylori suspension at the concentration 109CFU/ml at the 12 interval, triple. The mice of control groups were challenged with 0.5rnl saline solution at the concentration 0.9% at the 12 interval, triple. The mice of blank- control groups （n- 6） were no treatment. The animals were killed after 4th and 8th week of the last infection and gastric mucocal samples were taken for H. pylori histochemical examination, urease lest, and routine pathological HE and WS dye. Results： the infection rates of the mice in experimental groups at the 4th and 8th week after the last challenge were 100%. In all experimental group animals H. pylori was found to colonized on The surface of gastric mucosal cells and in the gastric pits, and the lamina propria of gastric mucosal was infihrated with chronic inflammatory cells. Gastric gland of 8W experimental group all animals atrophied. Conclusion： By using H. pylori suspension at high concentration of 1 × 10^9 for multiple times, the orally challenged Kuinming mice can be prepared as a stable and reliable H. pylori infection model. H. pylori can colonized in the gastric mucosa of the infected aninals, and inflammation re.actions can be seen.