杏丁注射液对血管内皮细胞HSP 72表达的影响

Effect of Xingding Injection on expression of heat shock protein 72 in vascular endothelial cells

目的:观察杏丁注射液对紫外线照射后血管内皮细胞热休克蛋白72(heatshockprotein72,HSP72)表达的影响。方法:采用体外培养的猪主动脉血管内皮细胞,以含浓度分别为0.1、0.5、1.0、5.0、10mg/ml的杏丁注射液的培养基培养72h后,用紫外线分别照射细胞30min,以Western-blot方法检测内皮细胞中的HSP72表达。结果:正常情况下检测不到HSP72表达,紫外线照射后血管内皮细胞HSP72有较强表达,杏丁注射液在0.1mg/ml浓度即有很明显的促进HSP72表达的作用,1.0mg/ml浓度时其作用达到高峰,再增加浓度并不能增加HSP72表达。结论:杏丁注射液能通过促进HSP72表达而保护血管内皮细胞在应激状态下免受损伤,这可能是其防治心脑血管疾病的重要机制之一。

To study the effect of Xingding Injection on the expression of heat shock protein 72 （HSP 72） in vascular endothelial cells after ultraviolet radiation. Methods： Porcine aortic endothelial cells were cultured for 72 hours in culture mediums with different concentrations （0. 1, 0.5, 1.0, 5.0, 10 mg/ml）of Xingding Injection. Ultraviolet radiation was administered to the cultured cells for 30 minutes. Western-blot assay was used to measure the expression of HSP 72 in the vascular endothelial cells. Results： There was no expression of HSP 72 in the cultured vascular endothelial cells without ultraviolet radiation, but there was some expression of HSP 72 after ultraviolet radiation. Xingding Injection of different concentrations could significantly improve the expression of HSP 72. The expression of HSP 72 in the vascular endothelial cells cultured in culture medium with 1. 0 mg/ml Xingding Injection was the highest, and there was no more increase of expression when the concentration was higher, instead the expression decreased. Conclusion：Xingding Injection can protect the vascular endothelial cells from injury during stress. It may be one of its mechanisms in preventing and treating cardio-cerebrovascular disorders.