骨髓间充质干细胞同种移植治疗急性心肌梗死的实验研究

Homogeneous transplantation of bone marrow mesenchymal stem cells for treatment of acute myocardial infarction in animal experiment

目的探讨骨髓间充质干细胞(MSCs)同种移植治疗急性心肌梗死的可行性。方法50只Wistar大鼠随机分为两组,对照组和移植组各25只。应用液氮冷冻法制作心肌梗死模型。移植组梗死部位注入经5-氮胞苷(5-aza)诱导和溴氮胞苷标记的大鼠MSCs,对照组梗死部位注入等容量DMEM液。分别于术前1天、术后1周和术后4周行超声心动图检查,以收缩期峰值速度(Vs)、左室舒张末容积(LVEDV)和左室射血分数(LVEF)为指标评价心功能。移植组于术后4周处死,移植心肌行溴氮胞苷免疫组化染色。结果对照组(n=13)和移植组(n=15)相比,术前及术后1周的心功能差异均无统计学意义(P>0.05),术后4周差异有统计学意义(P<0.05),术后4周移植组心功能明显优于对照组。移植部位免疫组化可见溴氮胞苷染色阳性细胞。结论经5-aza诱导的MSCs同种移植入大鼠急性心肌梗死模型的受损心肌后能存活并改善宿主的心功能。

Objective To investigate the feasibility of homogeneous transplantation of bone marrow mesenchymal stem cells （MSCs） for treatment of acute myocardial infarction. Methods Fifty rats were randomly separated into two groups including the control group （n=25） and the transplantation group （n=25）. Myocardial infarction were made by liquid nitrogen freezing. The rat MSCs induced by 5-azacytidine （5-aza） and marked by BrdU were injected into the infarcted sites of the transplantation group. The same volume of DMEM solution was injected into the infarcted sites of the control group. Echocardiography was applied preoperatively, one week and four weeks after operation. Cardiac function was evaluated by Vs, LVEDV and LVEF. The transplantation rats were executed four weeks after operation and the transplanted cardiac muscles were harvested for immunohistochemistry. Results The cardiac function measured preoperatively and one week after operation had no statistical difference between the control group （n=13） and transplantation group （n=15） （P〉0.05）. The cardiac function measured four weeks after operation had significant difference between the two groups （P〈0.05）. The transplantation group had better cardiac function than the control.Immunohistochemistry found BrdU-marked cells. Conclusion The rat MSCs induced by 5-aza and later implanted into the rat infarcted cardiac musle can survive and improve the host＇s cardiac function.