摘要
研究了提高酵母菌原生质体制备与再生率的技术路线:选用酵母菌对数生长中期,约培养9～12h(A600为0.5～1.0)的细胞,采用1.5%蜗牛酶和1.0%纤维素酶的混合酶液水解去除细胞壁,经特定条件,可获得98%的原生质体制备率,通过分离到含有0.01mol/LCaCl2和15%蔗糖的YEPDS再生培养基上培养,再生率可达95%以上。
A technical route to improve preparation and regeneration of yeast protoplasts was studied. The yeast was selected used at its medium logarithmic growth phase, cells at about 9-12 hours＇ culture （A600 at 0.5-1.0）, and adopted combined enzyme solution of 1.5 % helicase and 1.0 % cellulase to eliminate cell wall, under a specific conditions, 98% preparation rate of protoplasts can be obtained. Then they were separated into a regeneration YEPDS medium that contains 0.01 mol/L CaCIE and 15 % cane sugar to culture further, and the regeneration rate can reach over 95 % .
出处
《微生物学杂志》
CAS
CSCD
2005年第3期10-13,共4页
Journal of Microbiology
基金
福建省发展计划委员会项目(1996050)
福建省教育厅资助项目(JA010160)
关键词
酿酒酵母S-97
原生质体
制备
再生
Saccharomyces cerevisiae S-97
protoplast
preparation
regeneration
