同源基因型9L/Fischer344大鼠脑胶质细胞瘤模型的建立

Establishment of syngeneic 9L/Fischer344 rat brain glioma model

目的建立稳定的同源基因型9L/Fischer344大鼠脑胶质细胞瘤模型。方法采用立体定向技术,于Fischer344大鼠右侧尾状核区,接种定量的9L细胞;连续观察大鼠的生存状态、生存时间;大鼠死亡后,脑标本制片,行肿瘤组织病理学观察,胶质纤维酸性蛋白(GFAP)和S-100蛋白免疫组织化学检测。结果①在接种后45d观察期内,大鼠全部死亡。脑内分别接种5.0×102、1.0×105和1.0×1069L细胞的三组大鼠的中位生存时间分别为38、24、15d。②接种1.0×1059L细胞的大鼠,于接种后10、14、20dMRI增强扫描,脑内接种部位均见肿瘤,并呈持续生长。③组织病理学观察,肿瘤界线较明显,但无包膜,肿瘤细胞向周边脑组织内浸润,新生血管丰富,多见出血、坏死等特点。肿瘤细胞免疫组织化学GFAP和S-100蛋白染色阴性。结论同源基因型9L/Fischer344大鼠脑胶质细胞瘤模型成功建立,该模型稳定可靠,大鼠脑内肿瘤持续生长,符合恶性胶质肉瘤生物学特性。

Objective To estahlish the syngeneic rat brain glioma model of 9 L/Fischer344. Methods The 9L gliosarcoma cells were injected slereotaclicallv into the right caudate nuclear of syngeneic Fischer 344 rat. Thirtyfive rats were divided into 3 groups in random. Each rat received 5.0×10^2, 1.0×10^5 and 1.0 ×10^6 9L cells in a volume of 10-20μl, respet.tively. Implantation was followed by genera] observation. The survival time of each rat was recorded. MRI seanne（l on 10, 14 and 20 days after implantation for two rats received 1.0 ×10^5 9L cells. After the natural death, rat brains were sectioned and iaspected. The tumor-containing samples were prepared by haematoxylin-eosin（HE）, inmlunohistpchenliseal glial fibrillarv acidic protein （GFAP） and S-100 protein stains.Tumor pathologieal charaetedsties were investigated with nficmseope. Results ①All rats died within 45 clays after injection of 9L cells. The median sturvival tiures of 3 groups were 38, 24 and 15 clays, respectively. ②At 10, 14 and 20 days after implantation, brain tunmors were detected clearly by MRI with gadolinium dlethvlene triaminepentaac etieacid （Gd-DTPA）. The tumor sizes increased constantly during the olrservation. ③Demarcated massive tumors were found in the brains of all rats without extracerebral metastasis and extracranild extension. Central hemorrhage and neemsis was commonly seen in sanples. Morphology and microscopic structures for glioma, such as no encapsulation,intraparenehymal growth, neovaseulanity, were observed in HE stain sections. Immnunohistoehemistory GFAP and S100 protein stains of tumor cells were negative. Conclusion The syngeneie 9L/Fischer 344 rat brain glioma model is successfully established. After implantation, globular brain-tumor growth occurs constantly without extraeranial extension. The tuner pathological characteristics mimic human malignant glioma.