摘要
为了探讨人类ERMAP基因在红细胞分化发育过程中的作用,以Ara-C诱导K562细胞向红系方向分化,以十二氟波酯钠盐(TPA)诱导K562细胞向巨噬系方向分化,用荧光定量PCR检测上述过程中人类ERMAP基因表达量的变化。结果发现:终浓度为2.5×10-6mmol/L/L及1.0×10-6mmol/L/L的Ara-C可诱导K562细胞向红系方向分化,在此过程中人类ERMAP基因的表达量逐渐增加;终浓度为2.0×10-6mmol/L/L及1.0×10-6mmol/L/L的TPA可诱导K562细胞向巨噬系方向分化,在此过程中人类ERMAP基因的表达量无变化。结论:人类ER-MAP基因与红系分化增殖密切相关。
In order to investigate the potential of human ERMAP gene in erythroid cell differentiation, K562 cells were induced to erythroid lineage by Ara-C and to macrophage lineage by TPA, human ERMAP mRNA was detected by fluorescent quantitative PCR. The results showed that human ERMAP mRNA increased while K562 cells were induced to erythroid lineage after treatment with Ara-C at 2.5 ×10^-6 mmol/L/L and 1.0× 10^-6 mmol/L/L. Human ERMAP mRNA not changed while K562 cells were induced to macrophage lineage after treatment with TPA at 2.0 × 10^ -6 mmol/L/L and 1.0 × 10^-6 mmol/L/L. It is concluded that human ERMAP gene plays an important role in differentiation and proliferation of erythroid cells.
出处
《中国实验血液学杂志》
CAS
CSCD
2005年第4期553-556,共4页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目
编号30070797
