纳豆激酶液体发酵条件研究

Study on the optimization of nattokinase liquid fermentation by Bacillus natto

研究了纳豆芽孢杆菌(Bacillusnatto)液体发酵生产纳豆激酶的最佳发酵条件。摇瓶实验发现3%的大豆蛋白胨为氮源,2%的葡萄糖为碳源,添加Na2HPO40.6%、NaH2PO40.1%、CaCl20.02%和MgSO40.05%,调节pH为7.0￣7.2,接入体积比为2%的菌种悬液,在200r/min、37℃发酵60h,发酵液中NK的酶活力可达736IU/mL相当的尿激酶活力。

The optimization of Nattokinase liquid fermentation by Bacillus natto was investigated in this paper. The results indicated that when the fermentation medium was prepared with soya peptone 3%, glucose 2%, Na2HPO4 0.6%, NaH2PO4 0.1%, CaCl2 0.02% and MgSO4 0.05%, pH 7.0-7.2, and inoculums 2%, the yield of Nattokinase reached 736 IU Urokinase/ml after fermentation for 60 h at 37℃ under 200 r/min condition.