角蛋白在头皮中的分布及其在干细胞研究中的意义

The distribution of cytokeratin in the scalp and its signification in the research on hair follicle stem cells

目的:研究不同类型角蛋白(Cytokeratin,CK)在头皮及毛囊结构中表达分布情况。方法:将头皮病理切片和游离培养的毛囊使用不同类型角蛋白抗体进行免疫组化染色,检测角蛋白的分布情况。结果:CK15主要分布在毛囊隆突部,部分毛囊外根鞘最外层也有一定的表达;CK19主要分布在汗腺、毛囊外根鞘最外层和皮肤的基底层;CK16在表皮层和毛囊外根鞘均有阳性表达;CK10主要分布在头皮表皮的基底上层和皮脂腺腺泡的最外层。结论:不同类型角蛋白在头皮和毛囊结构中具有明显差异性,CK19和CK15阳性表达细胞可能具有干细胞特性。

Objective To investigate the expression and distribution of different cytokeratin in the scalp and hair follicle. Methods Immunohistochemistry was used to detect the expression and distribution of different cytokeratin in the scalp pathology slice and cultured hair follicle. Results The immunohistochemistry of scalp showed the CK15 mainly expressed in the bulge of the hair follicle,and some of the extra layer of outer root sheath.CK19 mainly expressed in the sweat gland,the out layer of outer root sheath and the basal layer of skin.CK16 expressed both in the epithelia and the outer root sheath.CK10 mainly distributed in the suprabasal epithelia and the out layer of sebaceous gland. Conclusion Great difference exists in the scalp and hair follicle for the distrition of the different cytokeratin.The stem cells could be the CK19 and CK15 positive cells.