42例卵巢肿瘤ICAM-1和S-100表达及意义

ICAM-1 and S-100 expression and significance of 42 ovarian tumors

目的:研究新生微淋巴管密度(MLD)与卵巢上皮性肿瘤细胞发生、发展的关系,并观察树突状细胞(DCs)在卵巢上皮性肿瘤组织的数量及分布,为临床评价卵巢肿瘤患者的肿瘤免疫能力和预后,抑制卵巢肿瘤淋巴转移提供实验依据。方法:采用扫描电镜、Envision免疫组织化学和Leica-Qwin图像分析技术观察42例卵巢上皮性肿瘤手术标本,数据经SPSS统计软件分析。结果:①卵巢上皮性肿瘤DCs的数量和S-100表达强度均明显减少和降低,与正常卵巢相比,有高度显著性差异(P<0·01)。②不同恶性程度的卵巢上皮性肿瘤DCs数量和S-100表达强度不同,与正常卵巢比较,DCs数量和S-100表达强度在卵巢上皮癌和交界性肿瘤均减少,而在良性上皮性肿瘤则无显著性差异。③VEGFR-3在卵巢肿瘤间质的淋巴管内皮细胞特异表达。④卵巢上皮癌组织中VEGFR-3阳性淋巴管密度高于良性和交界性卵巢肿瘤(P<0·05),而与组织学类型无关(P>0·05)。结论:随着卵巢上皮性肿瘤恶性程度的增高,DCs数量和S-100的表达减少,DCs可作为判断卵巢上皮性肿瘤恶性程度的指标;VEGFR-3的表达与卵巢上皮癌淋巴道转移密切相关。

Objective： To investigate the development of the newly micro - lymphatic density （MLD） and the distribution of deneritic cells （DCs） on the ovarian epithelial tumor in order to provide the approach to estimate the patient＇s immunity capability and prognosis and inhibite the lymphatic metastasis of ovary tumor. Methods ： Forty - two samples of ovarian epithelial tumor were observed with the scanning electron microscope, Envision immuno - histochemistry and Leica - Qwin image technology. All the results were analyzed with SPSS software. Results ： （ 1 ） With comparison to that of normal ovary, the distribution number of DCs significantly decreased on the ovarian epithelial tumor （P 〈 0. 01 ） . the expression intensity of S - 100 also existed significantly difference between the two groups （ P 〈 0. 01 ） .（2） The distribution number of DCs and the expression intensity of S - 100 varied with the malignant degree of ovarian epithelial tumor.Compared to those of normal ovary, they tended to decrease significantly in the ovarian epithelial carcinoma and the ovarian borderline epithelial tumor, while there existed no significance in the benign epithelial tumor. （3） Vascular endothelial growth factor receptor 3 （ VEGFR -3） was expressed highly in the lymphatic endothelial cell in the ovarian tumor. （4） the density of lymphatic vessel positive to VEGFR -3 protein in the ovarian epithelial carcinoma was more than that of the benign and boderline ovarian tumors （ P 〈 0. 05 ） , and it did not relate to the histological types. Conclusion： The results showed that DCs could be one of the indexes judging the malignant degree of ovarian epithelial tumor. The expression of VEGFR - 3 related to the lymphatic metastasis of ovarian tumor.