同种异体微小颗粒骨/磷酸钙骨水泥复合物修复骨缺损的实验研究

An experimental study on repairing bone defect with the compound of the allogeneic morselized bone and the calcium phosphate cement

目的:检测同种异体微小颗粒骨/磷酸钙骨水泥复合物修复骨缺损的效果,为其临床应用提供依据。方法:手术造成日本大耳白兔双侧桡骨中段12mm骨缺损模型,植入同种异体微小颗粒骨/磷酸钙骨水泥复合物为实验组,植入同种异体微小颗粒骨为对照组和不植入任何物质的为空白组。术后4周和8周分别行大体观察,X线摄片,组织学观察,骨缺损修复血管化观察,生物力学测定。结果:同种异体微小颗粒骨/磷酸钙骨水泥复合物组比同种异体微小颗粒骨组能更有效地修复骨缺损,空白组无骨愈合迹象;组织学观察示同种异体微小颗粒骨/磷酸钙骨水泥复合物组生成的骨优于同种异体微小颗粒骨组;生物力学测定证明同种异体微小颗粒骨/磷酸钙骨水泥复合物组的力学强度优于同种异体微小颗粒骨组。结论:同种异体微小颗粒骨能较好地修复骨缺损,同种异体微小颗粒骨/磷酸钙骨水泥复合物修复骨缺损的效果更加优异。

Objective：To probe the ability of repairing bone defect with the compound of the allogeneic morselized bone and the calcium phosphate cement, and to investigate the methods of morselized bone osteogenesis.Method： The animal models of bilateral radius bone defect were created by surgery in japan white big ear rabbits. The experimental group was implanted the compound of the allogeneie morselized bone. In the control groups, some were implanted the allogeneic morselized, and others were implanted nothing. The bone defect was observed by gross examination, X-ray examination, histopatholgieal examination,vascularization at 4,8 W,and was determined by biomechanics at 8W. Result：The experimental group repaired the bone defect more effectually than the group implanted the allogeneie morselized, but there was no change in the group implanted nothing.The quantidy and rate of the new formation bone in the examination group were lretter and faster than the group implanted the allogeneic morselized. The effect of biomeehanies in the examination group was statistically significant different with the group implanted the allogeneic morselized. Conclusion：The compound of the allogeneic morselized bone and the calcium phosphate cement formate bone more effectually. It＇s quantity, rate and biomechanics was excellent, and more ability of repairing the bone defect than the group implanted the allogeneie morselized.