油菜籽中主要硫甙的分离提纯

Separation and Purification of the Main Glucosinolate from Rapeseeds

用柱色谱方法从甘蓝型油菜籽中分离提纯了1-硫[(1Z)-3-羟基-1-[(磺酸基)亚氨基]-4-戊烯基]-1-硫代-β-D-葡萄糖钾盐(progoitrin)。用甲醇溶液提取菜籽中的硫甙,得到粗提物;粗提物经酸性氧化铝色谱柱与反相C18硅胶柱进一步分离提纯,得到纯品。对纯品进行紫外光谱、红外光谱、核磁共振氢谱、质谱和元素分析,测得的数据与文献值相符。用高效液相色谱测得硫甙提取物的纯度为99%。该方法操作简便,得到的硫甙样品纯度高,是一种有价值的硫甙提取方法,具有良好的应用前景。

A procedure for the preparative isolation of 1-S-[ （ 1 Z）-3-hydroxy-1-[ （sulfooxy） imino ]-4-pentenyl] -1-thio-β-D-glucopyanose, potassium salt （progoitrin） from Brassica oleracea is reported. The major steps in this procedure were： （ 1 ） extraction of glucosinolates with methanol from Brassica oleracea; （ 2 ） separation and purification of glucosinolates by chromatographic column on alumina support; and （3） a follow-up reversed-phase separation by octadecyl （C18） silica support yielding progoitrin as the main content of glucosinolate. The structure of progoitrin was identified on its physicochemical properties by ultraviolet absorption spectrometry, infrared absorption, ^1H nuclear magnetic resonance spectroscopy, mass spectrometry and elemental analysis. Spectroscopic data of the sample isolated were in agreement with those reported in the literature. The purity of progoitrin obtained was determined to be 99% by high performance liquid chromatography. The simplicity of the separation and purification procedure for glucosinolates and the high purity of progoitrin isolated would make the method an important reference for future research on glucosinolates and a favorable techniquefor future development.