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利用改进的Cap-trapper法构建拟斯卑尔脱山羊草(Ae.speltoides Tausch)全长cDNA文库 被引量：10

Construction of a Full-length cDNA Library of Aegilops speltoides Tausch with Optimized Cap-trapper Method
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摘要 Captrapper法是目前全长cDNA文库构建的重要方法之一。在引进、消化、吸收的基础上,通过设计引物,同位素检测,对末端转移反应条件控制等方面做了一些切实可行的改进,形成了一套简单易行的技术体系。利用改进的Captrapper方法,成功构建了小麦B基因组的可能供体种拟斯卑尔脱山羊草(Ae.speltoides)的全长cDNA文库。经综合评价,文库的全长比例达到89.6%,重组率为99%,库容量超过3.0×106cfu。 To discover new genes in a throughput manner,the cap-trapper method published previously was optimized for raising the efficiency in the construction of full-length cDNA library. Using the optimized protocol,we successfully constructed a full-length cDNA library of Aegilops speltoides,which contained 3.0 × 10^6 clones and more than 99% of plaques were recombinant phages. Sequence analysis results indicated that more than 89% of the clones were full-length.
作者 毛新国 孔秀英 赵光耀 贾继增
机构地区 中国农业科学院作物品种资源研究所
出处 《Acta Genetica Sinica》 SCIE CAS CSCD 北大核心 2005年第8期811-817,共7页
基金 国家重点基础研究发展规划项目(973计划)(编号:G19980102)~~
关键词 全长CDNA文库 Cap-trapper法 拟斯卑尔脱山羊草 同位素检测 小麦B基因组 full-length cDNA library Cap-trapper method Ae. speltoides
分类号 Q943.2 [生物学—植物学]
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参考文献12

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同被引文献165

引证文献10

二级引证文献78

Acta Genetica Sinica
2005年 第8期

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