利用高代回交和分子标记辅助选择建立水稻单片段代换系

Developing Single Segment Substitution Lines ( SSSLs ) in Rice (Oryza sativa L.) Using Advanced Backcrosses and MAS

以水稻品种华粳籼74为受体,以6个水稻品种为供体,通过高代回交和微卫星标记辅助选择相结合的方法,建立了水稻的一个单片段代换系群体。该群体由86个单片段代换系组成,其中52个在BC3F2中获得,34个在BC3F3中获得。每个单片段代换系只含有来自一个供体的一个染色体代换片段,而遗传背景与华粳籼74相同。这些单片段代换系的代换片段分布于水稻的12条染色体,代换片段的长度为1.5～56.3cM,平均长度为23.0cM。全部代换片段在水稻基因组上的覆盖率为57.1%。

A novel population consisted of 86 single segment substitution lines （SSSLs） were developed from advanced backcrosses between the recipient, Huajingxian -74 and six donors by microsatellite marker-assisted selection （MAS）. Fifty-two SSSLs were selected in BC3F2 ,and 34 others were selected in BC3F3. Every SSSL contains a single chromosome segment introgressed from one donor on the genetic background of Huajingxian 74. The substituted segments in SSSLs were distributed on 12 rice chromosomes. The estimated length of the substituted segments in SSSLs ranged from 1.5 cM to 56.3 cM with an average of 23.0 cM. Total of the substituted segments covered 57. 1% of the rice genome.