幽门弯曲菌的分离鉴定和超微结构

ISOLATION,IDENTIFICATION AND ULTRASTRUCTURE OF CAMPYLOBACTER PYLORIDIS

本文以MHB或MHC选择培养基经微需氧37℃培养,从116例胃镜检查患者胃粘膜活检标本中分离出Cp81株,阳性率为69.83%,其中各类胃炎71.76%(61/85)、消化性溃疡86.67%(13/15)、胃癌77.78%(7/9),7例正常胃粘膜未捡出Cp.分离株的茵落、形态和染色、生化反应及超微结构均符合Cp的特征.认为茵落特征、茵体形态和染色,以及快速尿素酶试验可作为常规鉴定Cp的依据.并讨论了影响Cp分离培养率的因素及超微结构上某些差异的可能原因.

Biopsy Specimens of human gastric mucosa of patients subjected to endoscopic examination were cultured microaerobicallY in MHB or MHC selective media at 37℃. Campylobacter pyloridis(Cp)was isolated in 8l out of 116 cases(69.83%), including 61 out of 85 patients with gastritis (71.76%), 13 out of 15 with peptie ulcer(86.67%)and 7 out of 9 with gastric carcinoma(77.78%). Nothing was detected in 7 cases of normal gastric mucosa. The features of the colony and microscopic morghology, phology, Stain and biochemical reactions and ultrastructure of the isolates corres-ponded with those of Cp. It is suggested that Cp can be routinely iden-tified by its colony characteristics, bacterial morphology, stain reactions and the rapid urease test. The factors influencing the isolation rate of Cp and reasons fo some probable differences in the ultrastructure are also discussed.