病毒唑体外抗腺病毒3型作用

Effect of Ribavirin on anti-adenoviruses 3 type in vitro

目的了解病毒唑在体外抑制腺病毒3型(ADV3)作用的效果和强度。方法采用细胞病变抑制实验观察病毒唑在人宫颈癌传代(Hela)细胞中对腺病毒3型的抑制作用。结果病毒唑半数中毒浓度(TC50)为2056·13μg/ml;抑制腺病毒3型的半数有效浓度(EC50)为53·03μg/ml,治疗指数(TI)为38·77;病毒唑对腺病毒3型的抑制作用存在量效关系;在感染后10h以内给药,病毒唑能够有效地抑制腺病毒3型的复制。结论病毒唑在Hela细胞中对腺病毒3型有明显的抑制作用。

Objective To study the inhibition action of the Ribavirin to Adenoviruses 3（ADV3）. Methods The antiADV3 effect of the ribaririn in Hela cell culture was observed by means of the inhibition of cytopathic effect. Results In cell culture, the Ribavirin was found to be a inhibitor of ADV3 in a concentration-dependent manner toxic concentration（TC50） of the Ribavirin was 2056.13μg/ml, the median effective concentration（EC50）of the Ribavirin to ADV3 was 53.03μg/ml,the selectivity index（TI）was 38.77, The Ribavirin could effectually inhibit replication of ADV3 when it was added in Hela cell within 10 hours after virus infection. Conclusion The Ribavirin has the evident inhibition actions to ADV3.