丙酯草醚对小鼠不同细胞DNA的损伤作用

Effect of new pesticide on DNA damage in different mice cells

目的探讨新合成的除草剂-丙酯草醚对小鼠离体骨髓细胞和精原细胞的DNA损伤作用。方法应用彗星技术测试不同浓度丙酯草醚对小鼠骨髓细胞和精原细胞DNA的损伤情况。结果丙酯草醚在(10g/L)浓度下与对照组比较差异有统计学意义(P<0·05)。丙酯草醚对精原细胞作用更敏感,且在10,100,500mg/L浓度下呈剂量-反应关系。结论新农药丙酯草醚对小鼠细胞有一定的损伤作用。

Objective To study the effects of a new pesticide on DNA damage of erythroblast cells and seminal cells in mice in vitro, Methods The comet assay was used to detect the DNA damage induced by a new pesticide compound at the different dose, Results Compared with the control group, the DNA migration rate were increase significantly at （10g/L）test doses to erythroblast cells（P〈0.05）, DNA migration of seminal cells（at test dose of 10,100,500mg/L）exhibited significant dose-effect relationship, The DNA damage in seminal cell was more sensitive than that of erythroblast cell. Conclusion The new pesticide could cause DNA damage of mice cells in vitro.