nm23-H_1、PCNA、p53蛋白在肺多形性癌组织中的表达及意义

目的:探讨nm23-H1、PCNA、p53蛋白在肺多形性癌组织中的表达及意义。方法:应用免疫组化SP法回顾性分析20例肺多形性癌组织中nm23-H1、PCNA、p53基因表达,并结合肺多形性癌的转移、侵袭力、肿块大小及预后等进行分析。结果:nm23-H1在肺多形性癌有无淋巴结转移中阳性率分别为25.0%和87.5%,差异有显著性(P<0.01),有无侵犯周围组织中阳性率各为37.5%和100%,差异有显著性(P<0.01);PCNA在肺多形性癌有无淋巴结转移中阳性率分别为66.7%和75.0%,差异无显著性(P>0.05),在有无侵犯周围组织中阳性率各为68.8%和75.0%,差异无显著性(P>0.05);p53在肺多形性癌有无淋巴结转移中阳性率分别为83.3%和25.0%,差异有显著性(P<0.01),在有无周围组织中阳性率各为68.8%和25.0%,差异有显著性(P<0.01)。Nm23-H1、p53基因表达与肺多形性癌淋巴结转移、侵袭力及预后有关。结论:m23-H1、P53基因能抑制肺多形性癌的转移及侵袭,而PCNA基因表达与肺多形性癌的转移和侵袭无明确相关性。

Objective： To observe the expression and role of nm23-H1,PCNA and P53 protein in lung polymorphic cancer tissue.Methods：The expression of nm23-H1,PCNA and P53 in lung polymorphic cancer were determined by immunohistochemistry, and combined the results with metastasis, invasiveness,tumor and prognosis of lung polymorphic cancer, then compated study. Results：The positive rates of nm23-H1,PCNA and P53 in lung polymorphic cancer tissues with and without lymphatic metastasis were 25.0% and 87.5% ,66.7% and 75.0% ,83.3%and 25.0% respectively, In lung polymorphic cancer with and without invasive peripheral tissue, the posivive rates were 37.5% and 100%,68.8% and 75.0%,68.8% and 25.0% resptctively, The nm23-H1 and P53 had obvious difference （P〈0.01 ）, but PCNA had not difference （P〉0.05）. The expression of nm23-H1 and P53 were closely related with the lymphatic metastasis、invasisveness and prognosis of lung Polymorphic cancer. Conciusions： nm23-H1 and P53 gene can effectively inhibit metastasis and invasiveness of lung polymorphic cancer, but the PCNA can not do it.