鼠脑缺血再灌注后星形胶质细胞的变化及补阳还五汤对其的影响

Effects of Buyanghuanwu decoction on astrocytes after cerebral ischemia and reperfusion

目的探讨沙土鼠脑缺血再灌注后星形胶质细胞(AS)的变化及补阳还五汤对其的影响。方法采用沙土鼠双侧颈动脉夹闭脑缺血模型,于脑缺血15min、再灌注24h和48h后,运用免疫组化技术观察星型胶质纤维酸性蛋白(GFAP)的动态表达。结果缺血再灌注24h后,GFAP免疫阳性反应达高峰,补阳还五汤可使GFAP免疫反应减轻;缺血再灌注48h后GFAP表达减弱,补阳还五汤可使其增强。结论补阳还五汤对脑缺血再灌注后星形胶质细胞的调节作用可能与脑缺血损伤后神经功能的恢复有关。

Objective To study the effects of Buyanghuanwu decoction on astrocytes after cerebral ischemia and reperfusion, Methods Cerebral ischemia model in gerbils was produced by ligating bilateral common carotid artery. The dynamic expressin of GFAP were determined by immunochemistry after cerebyal ischemia for 15min followed by reperfusion for 24 hours and 48 hours. Results GFAP positive reactions reached a peak after cerebral ischemia for 15min followed by reperfusion for 24 hours, Buyanghuanwu decoction decreased the expression. GFAP positive reactions decreased after cerebral ischemia for 15min followed by reperfusion for 48 hours,Buyanghuanwu decoction increased the expression. Conclusion The regulation of Buyanghuanwu decoction on astrocytes after cerebral ischemia and reperfusion may be related to repairing process after cerebral ischemia.