好氧颗粒污泥胞外聚合物提取方法研究

Study on the Extraction of Extracellular Polymer from Aerobic Granular Sludge

采用4种方法(热、超声、高压、碱处理)分别研究在不同的作用时间和方式下对好氧颗粒污泥EPS的提取效果。综合考虑各提取方法的提取效果及对于污泥细胞的破坏程度后,确定热处理(80℃,30min)和超声波提取(320W,40s)法均是可行的,其中热处理提取产物中多糖、蛋白质分别为30.3、8.1mg/gVSS,而超声波则分别为24.3、10.8mg/gVSS。好氧颗粒污泥EPS中多糖含量高于蛋白质,在热处理及超声波提取的产物中,多糖与蛋白质之比分别为3.7与2.3。

The effectiveness of extraction of extracellular polymeric substance （EPS） from aerobic granular sludge was studied by means of thermal, ultrasonic, autoclaving, and alkaline methods. After giving overall considerations to the effect of each extraction method and the damage to the sludge cell, it is concluded that both thermal treatment （80℃, 30 min） and ultrasonic extraction （320W, 40s） are effective, and the extracted polysaccharide and protein are 30.3, 8.1mg/gVSS and 24.3, 10.8mg/gVSS respectively by the thermal and ultrasonic method. In the EPS of aerobic granular sludge, the polysaccharide content is higher than protein, and the ratio of polysaccharide and protein is 3.7 and 2.3 respectively by thermal and ultrasonic method.