高效液相色谱法测定抗脑衰胶囊中葛根素的含量

Determination of Kangnaoshuai capsules by HPLC

目的:建立抗脑衰胶囊中葛根素的高效液相色谱(HPLC)含量测定方法。方法:用NovapakC18柱,流动相为甲醇水(25∶75),在250nm波长处检测。结果:葛根素浓度在10～100mg·L-1的范围内线性良好,葛根素的加样回收率为97.76%,RSD为1.27%(n=5)。结论:方法简便快速,精密度和稳定性良好,适合于抗脑衰胶囊的质量控制。

OBJECTIVE To establish an HPLC method for determination of Kangnaoshuai capsules. METHODS A Nova-pak C18 column was used,the mobiLe phase was methanol-water （25：75）,detection wavelength was 250 nm RESULTS The detection ranges were 10 - 100 mg·L^-1. The average recovery of puerarin was 97. 76%（RSD = 1.27%, n = 5）. CONCLUSION This HPLC method could be used for quaLity control in the Kangnaoshuai capsules.