甲基化特异性PCR检测胃癌p16基因甲基化及其临床意义

Detection of p16 gene methylation by methylation-specific PCR and its clinical significance in gastric cancer

目的初步探讨甲基化特异性聚合酶链反应(MSPCR)在胃癌临床诊断中的应用价值。方法选取47例胃癌患者的癌组织及配对的癌旁组织,11例远离肿瘤的正常胃黏膜组织。标本经一般处理,PCR扩增后,产物经电泳EB染色,紫外灯下观察。结果47例胃癌组织标本中,22例(46.2%)在p16基因的启动子区域呈现异常甲基化;47例癌旁组织标本中,28例(58.6%)也检测出甲基化存在;11例正常胃黏膜组织标本中均未检测出p16基因甲基化存在。结论MSPCR技术对胃癌患者组织标本中的异常甲基化检测具有高度特异性,是一项具有潜力的胃癌早期诊断新技术。

Objective To assess the clinical value of methylation- specific polymerase clain reaction （MSPCR） in the diagnosis of gastric cancer. Methods 47 surgical specimens of malignant tissue paired with adjacent tissue and 11 specimens of normal gastric mucosa far from the neoplasm taken from operated patients with gastric cancer were studied. The specimens were processed to extract DNA for PCR amplification. The PCR products, after electrophoresis and EB staining, were examined under ultraviolet light. Results Among the 47 specimens of gastric cancer tissues, 22 （46.2%） showed abnormal methylation in the promoter area of p16 gene, 28 （58.6%） showed abnormal methylation in the tissue adjacent to gastric cancer. But no methylation was found in the 11 specimens of normal mucosa. Conclusion MSPCR is highly specific for detecting abnormal methylation in gastric cancer, and can be a promising new technique for early diagnosis of gastric cancer.