摘要
目的检测胃肠道肿瘤患者外周血中CD28+T细胞数量及原代细胞表面B7表达水平,以探讨胃肠道肿瘤患者共刺激通路是否异常。方法采用流式细胞术检测胃肠道肿瘤患者外周血中CD28+T细胞与胃肠道肿瘤患者原代细胞B7分子表达,并与胃肠道良性疾病进行比较。结果胃肠道肿瘤患者CD28+T细胞数明显低于对照组(P<0.01);其原代细胞表面B7-1、B7-2表达水平低于对照组(P<0.01)。结论胃肠道肿瘤患者外周血T细胞低表达CD28分子,胃肠道肿瘤细胞低表达B7分子,从而影响B7-CD28共刺激通路,使T细胞不能有效地清除肿瘤。
Objective To investigate costimulatory pathway of gastrointestinal carcinoma patients, we examined peripheral CD28^＋T lymphocytes and B7 molecules expression on the histiocytes of carcinoma in patients with gastrointestinal cancer. Methods Flow cytometry was used to analyses CD28 and B7 molecules expressed on T lymphocytes and primary gastrointestinal carcinoma cells respectively. Results CD28^＋T lymphocytes in gastrointestinal carcinoma patients were lower than those in control group（P＜0.01）. B7 molecules expressed on primary gastrointestinal carcinoma cells were lower than those in control group（P＜0.01）. Conclusion Peripheral T lymphocytes in gastrointestinal carcinoma patients express low level of CD28 molecule. Primary gastrointestinal carcinoma cells express low level of B7 molecules. Costimulatory pathway dysfunction in stomach tumor patients results in T lymphocytes can not eliminate the tumor cells.
出处
《检验医学与临床》
CAS
2005年第3期106-107,共2页
Laboratory Medicine and Clinic
