黄芪多糖对兔动脉粥样硬化血管内皮细胞功能的影响

The effect of astragalus polysaccharide on the function of endothelial cells in atherosclerosis rabbits

目的:探讨黄芪多糖对新西兰兔动脉粥样硬化内皮细胞功能的影响。方法:将40只雄性新西兰兔随机分为4组,每组10只,对照组喂普通饲料;模型组:普通饲料加质量分数为1.5%的胆固醇喂养;黄芪多糖(APS)组:普通饲料加质量分数为1.5%的胆固醇喂养,同时腹腔注射APS(12.0mg/kg);卡托普利组:普通饲料加质量分数为1.5%的胆固醇喂养,同时灌喂卡托普利(15.0mg/kg);10周后,测定各组空腹血清或血浆三脂酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、一氧化氮(NO)、内皮素-1(ET-1)、丙二醛(MDA)、C反应蛋白(CRP)含量以及超氧化物歧化酶(SOD)活性,并计算主动脉内膜粥样斑块面积。结果:模型组与对照组比较,TG、TC、MDA、ET-1、NO、CRP明显升高(P<0.01),HDL-C/TC比值、SOD活性明显下降(P<0.01),主动脉内膜粥样斑块面积较大(P<0.01);卡托普利组与模型组比较,各项指标无明显差异;而APS组与模型组比较,TG、CRP、NO、MDA、ET-1明显下降(P<0.01),TC无明显变化,HDL-C/TC比值、SOD活性明显升高(P<0.01),主动脉内膜粥样斑块面积明显减少(P<0.01)。结论:黄芪多糖对动脉粥样硬化有明显的预防和治疗作用,可能与抗氧化、免疫调节、保护血管内皮细胞有关。

Objective： To investigate the effect of Astragalus Polysaccharide （APS） on the function of endothelial cells in atherogenesls rabbits. Methods：Foty healthy New Zealand white rabbits were randomly divided into four groops. Control groop was fed with normal diet for 10 weeks ,model groop was fed with 1. S% cholesterol diet plus normal diet;APS groop was fed with 1.5% cholesterol plus 12.0mg/kg APS plus normal diet;Captopril groop was fed with 1. 5% cholesterol plus 15. 0mg/kg Captopril plus normal diet. After ten weeks＇ experiment, the concentration of each group＇s Triglyceride, Total Cholesterol, High Density Lipoprotein Cholesterol, C Reactive Protein, Nitric Oxide, Maleic Dialdehyde and Endothelin-1 were measured, so was the activity of superoxide dismutase. Results：Compared with the normal control group ,model groups＇ TG ,TC, NO ,MDA ,CRP and ET-1 increased significantly （P〈0. 01 ）, and the activity of SOD and the ratio of HDL-C/TC remarkably decreased（P〈0.01）. Compared with the model group,each index did not have significant change in captopril groop. But compared with the model group ,the APS group＇s TG ,NO ,MDA ,CRP and ET-1 significantly decreased （P〈0.01）, while the activity of SOD and the ratio of HDL-C/TC remarkably increased （P〈 0. 01）, and TC did not show significant change. Conclusion： APS can effectively accentuate the activity of SOD against oxidation, regulate immunity and protect endothelial function against atherogenesis,so APS is a potential drug in treating and preventing atherogenesis.